Endonuclease IV, E. coli

Description:
Endonuclease IV (E. coli) is a class II apurinic/apyrimidic (AP) enzyme that cleaves 5' to an AP site by hydrolysis, leaving a hydroxyl group at the 3' terminus and a deoxyribose 5'-phosphate at the 5'-terminus. Endo IV can be used in vivo to repair free radical damage in DNA.

Application:
- DNA structure research
- Studies of DNA damage and repair
- Single cell electrophoresis (comet assay)
- Anti-tumor drug studies

Source:
E. coli cells with a cloned nfo gene.

Unit Definition:
One unit of the enzyme relaxes 1µg of partially depurinated, covalently closed supercoiled plasmid DNA in 30 min at 37°C

Storage Buffer:
Supplied in 50% Glycercol, 50 mM Tris-HCl (pH 7.5), 1mM DTT, 0.1 M NaCl, 0.1% Triton X-100

10x Endonuclease IV Buffer
1M NaCl
500 mM Tris-HCl
100 mM MgCl₂
10 mM DTT pH 7.9 @ 25°C

Quality Control:
Endonuclease IV is tested in degradation of dsDNA and is free of detectable RNase and double-stranded exonuclease activities.

Recommended Storage Condition: -20°C

Reference:
1. Izumi, T. et al. (1992) J. Bacteriol. 174, 7711.
2. Levin, J.D. and Demple, B. (1996) Nucleic Acids Res. 24, 885.