Čeština
Polski
Endonuclease VIII, E. coli
Endonuclease VIII from E. coli acts as both an N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating an apurinic (AP site).
Description
Endonuclease V, (Endo V) is a 3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.
Endonuclease V is also active toward abasic sites and urea sites, base pair mismatches, flap and pseudo Y structures, and small insertions/deletions in DNA molecules. The cleavage site generated by Endonuclease V is at the second phosphodiester bond 3' to a lesion.
Applications
High-throughput methods for mutation research
Studies in mutagenesis and DNA repair
Mismatch cleavage
Genotyping
Source
E. coli
Purity
> 95 % as determined by SDS-PAGE
Storage
-80 Avoid repeated freeze-thaw cycles.
Unit Definition
One unit of T4 Endonuclease V converts 1 µg of UV-irradiated plasmid DNA from covalently closed circles to nicked form in 30 minutes at 37°C.
ENV-100
New
Comments (0)
No customer reviews for the moment.
8 other products in the same category:
MutS Protein (Thermus aquaticus)
The MutS DNA mismatch protein recognizes heteroduplex DNAs containing mispaired or unpaired bases.
Endonuclease III (Nth)
Endonuclease III (Nth) protein from E. coli acts as both N-glycosylase and a AP-lyase. The N-glycosylase activity releases damaged pyrimidines from double-stranded DNA, generating a basic (AP site).
Human Alkyl Adenine DNA Glycosylase
Human Recombinant Alkyl Adenine DNA Glycosylase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 306 amino acids (1-298 a.a.) and having a molecular mass of 33.9kDa (Molecular weight on SDS-PAGE will appear higher).
Viewed products
DNA Fragment Size Analysis Kit
MCLab’s DNA Fragment Size Analysis Kit offers fast and reliable separation, sizing and quantification of DNA or RNA libraries utilizing Capillary Electrophoresis technology, which can separate molecules with a single base difference.
Tn5 Transposase
origin: E. coli carrying the Tn5 gene
random transposon insertion into DNA
requires 19-bp ME sequence
Endonuclease V, (Endo V) is a 3'-endonuclease involved in DNA repair, which initiates removal of deaminated bases from damaged DNA, including uracil, hypoxanthine, and xanthine.