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Description:
Sticky End Ligation Master Mix is a specially formulated 2X master mix designed for fast and efficient ligation of DNA fragments with sticky (cohesive) ends featuring 2–4 nucleotide overhangs. It contains T4 DNA ligase and unique additives that significantly enhance the efficiency of joining typical overhang-ended DNA fragments.
The master mix can ligate the following types of DNA fragments:
The image shows the result of ligating DNA fragments with sticky ends using Sticky End Ligation Master Mix for 1–10 minutes at room temperature. The ligated plasmids were transformed into DH-5α competent cells and cultured overnight on LB medium plates at 37 °C.
Features:
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Extra-fast ligation – sticky-end fragment ligation in just 2–4 minutes at room temperature (20–25 °C)
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User-friendly – ready-to-use 2X master mix for simple reaction setup – just mix DNA fragments with the mix
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High efficiency – excellent performance even with low DNA amounts
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Time-saving – optimized transformation protocol saves time and simplifies lab handling
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Compact reaction volume – suitable even with limited sample quantities
Applications:
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Ligation of double-stranded DNA fragments with 2–4 nucleotide overhangs (sticky ends)
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Cloning, vector preparation, or adapter ligation for downstream applications
Product sizes:
50 reactions (Cat. 40103S)250 reactions (Cat. 40103L)
40103S
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