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MCMag™ NGS Library Clean Up & Size Selection Kit
MCMag™ Library Purification kit is a highly efficient purification system with size selection for NGS library after PCR enrichment.
Description
The MCMag PCR purification system utilizes solid-phase reversible immobilization (SPRI) paramagnetic bead technology for PCR amplicon purification. The MCMag beads are pre-formulated with an optimized buffer to selectively bind DNA fragments of 100 bp and larger. Excess salts, enzymes, primers and nucleotides can be removed through a simple washing procedure. The MCMag PCR Purification system is fully adaptable to automation.
Applications
- PCR
- Sequencing
- Fragment Analysis
- Genotyping
- Cloning
- Primer Walking
Material Supplied
- MCMag PCR Purification beads
- Store at 4°C upon arrival, for up to 18 months
- Shake the reagent well to a homogenous appearance before use
Specifications
Table 1. Available product sizes
| MCMag PCR Purification |
Catalog Number |
|
5 mL |
MCPP-5 |
|
60 mL |
MCPP-60 |
|
450 mL |
MCPP-450 |
Table 2. Number of PCR reactions purified with 96- and 384-well formats
|
Product Size |
|||
|
PCR reaction volume (µL) 96-well format |
5 mL |
60 mL |
450 mL |
|
10 |
278 rxns |
3332 rxns |
25000 rxns |
|
20 |
139 rxns |
1666 rxns |
12500 rxns |
|
50 |
56 rxns |
667 rxns |
5000 rxns |
|
100 |
28 rxns |
334 rxns |
2500 rxns |
|
PCR reaction volume (µL) 384-well format |
|||
|
5 |
556 rxns |
6667 rxns |
50000 rxns |
|
7 |
397 rxns |
4762 rxns |
35714 rxns |
|
10 |
278 rxns |
3333 rxns |
20000 rxns |
|
14 |
198 rxns |
2381 rxns |
17857 rxns |
Materials Not Supplied
- Reaction Plate
- McBead Magnetic Plate
- Plate Seals, Adhesive or Heat
- Wash Solution (70% ethanol)
- Elution Buffer
- Water
- Tris-Acetate (10 mM pH 8.0)
- TE Buffer (10 mM Tris-Acetate pH 8.0, 1 mM EDTA
MCPP-5
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