qPCR-HS Mix EvaGreen®

Description:
qPCR-HS Mix EvaGreen® is a ready-to-use reaction mix for real-time Hot Start PCR with EvaGreen® dye, supplied as a 2x concentrate. It contains Taq DNA polymerase blocked with a monoclonal antibody (RUN-HS), which is activated during the initial denaturation step of the PCR. The mix contains all components required for qPCR except the DNA template and primers. According to the manufacturer, it is optimized for high specificity and recommended for High Resolution Melting (HRM) analysis.

Features:

• Ready-to-use reaction mix for real-time Hot Start PCR
• 2x concentrated
• Detection with EvaGreen® dye
• Taq DNA polymerase blocked with a monoclonal antibody (RUN-HS)
• Polymerase activation during initial denaturation
• Contains all qPCR components except DNA template and primers
• According to the manufacturer, up to 7 repeated freeze-thaw cycles do not affect the activity of the product
• Storage at -20 °C
• For research use only

Applications:

• Real-time Hot Start PCR with EvaGreen® detection: amplification of a DNA template with fluorescence monitoring
• High Resolution Melting (HRM) analysis
• Confirmation of reaction specificity by melt curve analysis, which the manufacturer recommends
• Use with instruments that perform fluorescence signal correction: the manufacturer recommends adding ROX reference dye (not included)

Kit contents:

• 2x qPCR-HS Mix EvaGreen®
• Ultrapure water

Specification: