MC Lab (9)

CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins.

EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC).

RNase H is an endoribonuclease which degrades the RNA strand of RNA/DNA hybrid molecules.

Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3’ hydroxyl terminus of single or double stranded DNA molecules.

The 2X MCAmp library amplification master mix has been specially formulated to provide accurate and robust amplification of NGS (next-generation sequencing) library fragments.

1 mg, 1 mg/ml

Inhibitor RNazowy to kwasowe białko o masie 52 kDa, które jest silnym inhibitorem niekonkurencyjnym rybonukleaz typu trzustkowego, takich jak RNaza A, RNaza B i RNaza C.

synthesis at higher temperatures

reduced RNase H activity

addition of deoxynucleotides to the 3′ hydroxyl terminus

origin: E.coli (gen from Z. albicollis)

purity > 95 %

TWEEN 20 helps to prevent non-specific antibody binding in enzyme immunoassay as a washing agent (e.g. Western blots and ELISAs). In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v.

D-(+)-Glucose is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. Glucose is involved in a detrimental process in cells called glycation. Glucose is used as a supplement for cell culture and in numerous cellular processes and molecular biology applications.

Benzonase endonuclease from Serratia marcescens, can be used to degrade all forms of DNA and RNA while having no proteolytic activity.

MCMax Cell-Free DNA Extraction Kit został zaprojektowany do oczyszczania DNA pozbawionego komórek (cfDNA) z próbek płynu ustrojowego o objętości 0,2–10 ml. 

10 μg, 100 ug/ml

Adenozyno-5'-trifosforan sulfurylaza jest oczyszczana z E. coli zawierającej gen sulfurylazy adenozyno-5'-trifosforanu z drożdżami.

CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate) is a zwitterionic detergent used to solubilize proteins. Due to its low light absorbance in the ultraviolet region of the electromagnetic spectrum, CHAPSO is useful for researchers monitoring ongoing chemical reactions or protein-protein binding with UV/Vis spectroscopy.