MagnifiQ™ CiTi Converter DNA Methylation Kit

Description:
MagnifiQ™ CiTi Converter DNA Methylation Kit is a complete kit for bisulfite conversion and purification of converted DNA for high-throughput methylation analysis. Purification uses magnetic beads and a magnetic stand intended for manual operation. The kit is designed to provide complete conversion of unmethylated cytosines and to minimize template degradation and loss of DNA during treatment and clean-up. The manufacturer also states high-throughput, complete conversion of GC-rich DNA, a simple and efficient magnetic purification method, and eluted ultra-pure DNA suitable for molecular analyses.

Features:

• Complete kit for DNA conversion and purification of converted DNA
• Purification with MQBB magnetic beads
• Designed for manual operation with a magnetic stand (not included)
• Input DNA amount 500 pg to 2 µg, optimally 200–500 ng
• Elution volume 15–40 µl (Tris buffer or ultrapure water)
• C/T conversion reagent supplied as a solid in a light-protecting amber tube, one tube is designed for 10 separate conversions
• According to the manufacturer, complete conversion of GC-rich DNA
• According to the manufacturer, minimized DNA degradation and loss
• For research use only

Applications:

• Bisulfite conversion of DNA: conversion of unmethylated cytosines for DNA methylation research
• Purification of converted DNA with magnetic beads
• High-throughput DNA methylation analysis
• Preparation of ultra-pure DNA for downstream molecular analyses

Kit contents:

• C/T conversion reagent
• D dilution solution
• MQBB magnetic beads
• G binding solution
• A1 wash solution
• DS desulphonation solution
• Tris buffer
• Ultrapure water