Genomic Mini AX Plant Spin

Description:
Genomic Mini AX Plant Spin is an increased efficiency kit for genomic DNA purification from plant tissue using minicolumns. It processes up to 20 mg of dried, powdered plant material or up to 100 mg of fresh or frozen cut tissue. The sample is lysed with LS lysis suspension and Proteinase K, after centrifugation the supernatant is applied to the Mini AX Spin column, the DNA is washed with W1 and W2 wash solutions and eluted with E elution buffer. The kit also includes N neutralizing buffer, which enables secure DNA storage in 10 mM Tris-HCl, pH 8.5, and T solution for testing the functionality of the elution buffer.

Features:

• Genomic DNA isolation from plant tissue on minicolumns (Mini AX Spin)
• Binding capacity 15 µg DNA
• Sample size: up to 20 mg of dried plant tissue or up to 100 mg of fresh or frozen plant tissue
• Elution volume 100–150 µl
• Elution solution: E elution buffer (without EDTA)
• Includes T solution for testing the functionality of the E elution buffer
• Includes N neutralizing buffer for secure storage of eluted DNA
• Optional RNA removal with RNase (not included)
• For research use only

Applications:

• Genomic DNA isolation from dried plant tissue: up to 20 mg of powdered material
• Genomic DNA isolation from fresh or frozen plant tissue: up to 100 mg of cut tissue

Kit contents:

• Mini AX Spin columns (storage at 2–8 °C)
• 2 ml tubes
• LS lysis suspension
• W1 first wash solution
• W2 second wash solution
• E elution buffer (without EDTA, storage at 2–8 °C)
• N neutralizing buffer
• T solution (storage at 2–8 °C)
• Proteinase K (storage at 2–8 °C)

Specification: