50% Triton X-114
TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
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Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.
Description
Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.
Application
Used as a blocking agent in Northern, Southern and dot blot hybridizations
Added to buffers for nick translation, polymerase reactions and ligations
Common additive for PCR amplifications, footprinting and gel shift assays
Enhances enzyme activity in restriction digests
Concentration
100 mg/ml
Recommended Storage Condition
BSA should be stored at –20°C.
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TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).
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Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.
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