10% SDS (Sodium Dodecyl Sulfate)

EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC).

TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis¹. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. 

The β-galactosidase substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic stain for β-gal activity, and is commonly used to distinguish between recombinants and nonrecombinants by lacZ α-complementation with appropriate vectors and hosts.

25% Triton X-100 is a commonly used detergent in laboratories.

5M solution of betaine in MilliQ H2O. DNase, RNase free.

HEPES Solution is a biological buffer useful in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.

The Super-DITM Formamide is the recommended sample loading buffer for all ABI sequencers.

Ammonium acetate is a widely used reagent in molecular biology and chromatography. Suitable applications include the purification and precipitation of DNA and protein crystallization. Ammonium acetate is commonly used in HPLC and MS analysis of various compounds, such as oligosaccharides, proteins, and peptides.

Acetylated BSA is used as a carrier protein and an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is vital. The acetylation process inactivates any trace of active nuclease activity. While the acetylation process does change BSA's binding characteristics and makes it inhibitory in PCR reactions, it is normally added to restriction digests.

The loading dye increases the density of the sample and adds colours to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon.

Used to select for ampicillin resistance in mutated and transformed cells.

TRITON X-114 is used in biochemical applications to solubilize and separate proteins due to its low cloud point (23 °C).

miRNA purification

Efficiency up to 80%

Removal of tRNA, 5S RNA

Purification of PCR fragments >80 bp
Removal of primers <30 nt
Manual/automated processing

DNA and RNA concentrator

Binding capacity: 10 µg DNA per prep

Removal of unwanted components