TWEEN 40

Brij-35 (30% Solution) is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or as a surfactant in various HPLC applications.

EDTA, 0.5 M, pH 8.0, is a sterile-filtered solution of 0.5 M EDTA in H₂O treated with diethyl pyrocarbonate (DEPC).

SDS, sodium dodecyl sulfate is an anionic surfactant. SDS is commonly used in laboratory as a component of buffer for cell lysis, cell lysis during DNA extraction, and mostly in SDS-PAGE running buffer. Indeed, SDS is an anionic detergent applied to protein samples to linearize proteins and to impart a negative charge to linearized proteins.

Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria.

Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.

A routine use of agarose is ideal for daily analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). It has low ethidium bromide and SYBR Green background staining.

DEPC-treated Water is deionized, high quality, molecular biology grade water.

MCLAB’s 7M Guanidine-HCl Solution is a ready-to-use solution of guanidine hydrochloride, which can be easily diluted and pH-adjusted to any concentration below 7M.

Reported use in all phases of detergent compounding and aqueous processing in the textile and paper industries, in industrial metal cleaners, acid cleaners, floor cleaners, detergent sanitizers and waterless hand cleaners.

LB Agar plates contain 1.0% Tryptone, 0.5% yeast extract, 0.5% NaCl, 1.5% agar.

The β-galactosidase substrate X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside) is a chromogenic stain for β-gal activity, and is commonly used to distinguish between recombinants and nonrecombinants by lacZ α-complementation with appropriate vectors and hosts.

Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.

TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA and RNA.

HEPES Solution is a biological buffer useful in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.

Bovine Serum Albumin (BSA) has many uses as a carrier protein and as a stabilizing agent in enzymatic reactions. MCLAB’s Ultrapure BSA is a "non-acetylated" BSA, pure enough to use when the integrity of DNA or RNA is essential. It has been tested for DNase, RNase, endonuclease, protease, peroxidase, and alkaline phosphatase activity, and assayed for fluorescence background.