Linear acrylamide is widely used as one of the coprecipitants which are inert substances used to aid recovery of nucleic acids before alcohol precipitations.
Description:
Linear acrylamide is widely used as one of the coprecipitants which are inert substances used to aid recovery of nucleic acids before alcohol precipitations. It is chemically synthesized and is the most ideal for use upstream of RT-PCR without biological material contaminating. It has been shown to be able to separate picogram amounts of DNA fragments larger than 20 base pairs from unincorporated nucleotides and from most of the oligonucleotide primers.
The Coprecipitant Linear Acrylamide is not interfering with A260/280 readings.
Applications:
Recommended Storage Condition: -20°C
Low concentration solutions of DNA are prone to DNA degradation or other loss. It is critical that optimal methods are employed for DNA suspension and long-term storage.
CHAPSO (3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate) is a zwitterionic detergent used to solubilize proteins. Due to its low light absorbance in the ultraviolet region of the electromagnetic spectrum, CHAPSO is useful for researchers monitoring ongoing chemical reactions or protein-protein binding with UV/Vis spectroscopy.
TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis1. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis.
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Used to select for ampicillin resistance in mutated and transformed cells.
IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under the control of the lac operon.
Brij-35 (30% Solution) is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or as a surfactant in various HPLC applications.
The loading dye increases the density of the sample and adds colours to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.
TWEEN 20 helps to prevent non-specific antibody binding in enzyme immunoassay as a washing agent (e.g. Western blots and ELISAs). In this major application, it is dissolved in Tris-Buffered Saline or Phosphate buffered saline at dilutions of 0.05% to 0.5% v/v.
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Ammonium acetate is a widely used reagent in molecular biology and chromatography. Suitable applications include the purification and precipitation of DNA and protein crystallization. Ammonium acetate is commonly used in HPLC and MS analysis of various compounds, such as oligosaccharides, proteins, and peptides.
25% Triton X-100 is a commonly used detergent in laboratories.
Contains 100% active components and is a mixture of organic non - silicone polypropylene based polyether dispersions. Can be sterilized repeatedly.
Linear acrylamide is widely used as one of the coprecipitants which are inert substances used to aid recovery of nucleic acids before alcohol precipitations.
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