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DNA Fragmentation & A-tailing Enzyme Mix
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Description:
Cas12a2, a Type V CRISPR/Cas nuclease, cleaves the target RNA following target RNA recognition by the complementary crRNA. Cas12a2 processes its own crRNAs. The PFS requirement for Cas12a2 is 5′-GAAAG-3′. Cas12a2 can indiscriminately degrade single-stranded DNA (ssDNA), double-stranded DNA (dsDNA), and single -stranded RNA (ssRNA) as collateral activities once sensing correct crRNA-guided RNA binding. These properties enable Cas12a2 as a single-effector nuclease efficiently and sensitively for RNA detection. SuCas12a2 from Sulfuricurvum sp. PC08-66 is a programmable nuclease guided by a 42-44 nt crRNA and can be applied in RNA detection and genome editing.
The enzyme's purity is greater than 95%, as determined by SDS-PAGE and FPLC.
Activity: For the cleavage assay: 20 µl reaction, including SuCas12a2 Enzyme, crRNA, target RNA and 1x reaction buffer, preheated at 37°C for 15 min. Then for the ssDNA probe cleavage assay as Fig.1 below, added 100 nM ssDNA 5’FAM probe, incubated at 37°C for 30 min; For the plasmid cleavage assay as Fig. 2 below, added 7 nM pUC19 plasmid, incubated at 37°C for 25 min, and stop reaction with 1 µl Proteinase K (800 U/ml).
Application:
- RNA Detection
- CRISPR Diagnostics
- Genome Editing
Source:
Cas12a2 gene from Sulfuricurvum sp. PC08-66 (SuCas12a2, UniProtKB: A0A0C2W1L1) expressed in E. coli.
Storage:
-20°C
Size:
200 pmol, 200 µl (1 µM) (cat.n. Cas12a2-100)
1 nmol, 200 µl (5 µM) (cat.n. Cas12a2-200)
10X Cas12a2 Reaction Buffer 200 µl (cat.n. CAS12A-BUF-100-A2)
10X Cas12a2 Reaction Buffer 1 ml (cat.n. CAS12A-BUF-200-A2)
Cas12a2-100
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Viewed products
PFS 5′-GAAAG-3′
Origin: Sulfuricurvum sp. PC08-66
Purity > 95%