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DNA Fragmentation & A-tailing Enzyme Mix
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Description:
Cas12a (also referred as Cpf1) nuclease, belongs to the Class 2, Type V CRISPR system. dCas12a from Lachnospiraceae bacterium (strain ND2006) is a catalytically inactive LbCas12a (D832A, with an Asp832→Ala substitution). The protein can be programmed with a gRNA and directed to bind at a desired sequence of DNA.
The enzyme's purity is greater than 95%.
Application:
- Genome Editing
- DNA Imaging
Source:
dCas12a( D832A) from Lachnospiraceae bacterium (strain ND2006) expressed in E. coli.
Storage:
-20°C
Size:
200 pmol, 200 µl (1 µM) (cat.n. dCAS12A-100)
10X Cas12a Reaction Buffer 200 µl (cat.n. CAS12A-BUF-100-D)
10X Cas12a Reaction Buffer 1 ml (cat.n. DCAS12A-BUF-200)
dCAS12A-100
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LbCas12a (D832A, with an Asp832→Ala substitution)
Origin: Lachnospiraceae bacterium (strain ND2006)
Purity > 95%