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DNA Fragmentation & A-tailing Enzyme Mix
Fast and simple fragmentation
Fragment size easily controlled by incubation time
Ideal for NGS library preparation
Description:
FEN1 (AFU) is a recombinant flap endonuclease-1 (FEN-1) protein from the E. coli containing FEN1 Gene of hyperthermophilic Archaea strain,Archaeoglobus fulgidus. The FEN1 removes 5'-overhanging flaps in DNA repair and processes the 5'-ends of Okazaki fragments in lagging strand DNA synthesis.
Application:
FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis.
Source: E coli
Concentration: 0.5 mg/ml
Supplied In:
50 mM Tris-HCl (pH 8.0)
50 mM NaCl
1 mM DTT
0.1 mg/ml BSA
50% (v/v) glycerol
Supplied With:
10x Taq PCR Buffer (no dNTP)
Recommended Storage Condition: -20°C
AFU-100
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Fast and simple fragmentation
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Ideal for NGS library preparation
125 ug, 0.5 mg/ml