Plasmid Purification Beads (RNA Depletion)

Description:

The magnetic beads for plasmid purification from the American company BioDynami combine BioDynami's patented chemistry with the unique properties of SPRI beads (Solid Phase Reversible Immobilization). These beads effectively eliminate RNA contamination from isolated plasmid DNA samples. Additionally, they remove undesirable components such as salts, dNTPs, proteins, enzymes, and other impurities. The purified plasmid DNA obtained using this method is ideal for downstream applications such as enzymatic digestion, transformation, transfection, molecular cloning, and more. These beads provide an effective and cost-efficient tool for removing bacterial RNA during routine plasmid purification.

The image visualizes a comparison of samples before and after using magnetic beads for plasmid DNA purification. The left panel shows significant RNA contamination in the unpurified sample (-), while the right panel demonstrates effective RNA removal after purification (+). The use of magnetic beads results in clean and high-quality DNA, ready for further analysis.

Workflow:

First, the plasmid DNA is mixed with magnetic beads that specifically bind to the DNA, while RNA and other impurities remain in the solution. The beads with bound DNA are then separated from the rest of the solution using a magnet, effectively removing RNA and impurities. Next, the beads are washed with a wash buffer to remove any remaining contaminants. Finally, the purified plasmid DNA is eluted from the beads, resulting in highly purified plasmid DNA.

Features:

• Effective depletion of bacterial RNA by RNase
• High recovery rate of plasmid DNA by magnetic beads
• Removal of unwanted components and impurities
• Simple and fast beads-based protocol

Package size:

40 reactions (Cat. No. 40059S)
200 reactions (Cat. No. 40059L)