DNA Normalization Beads (NGS, PCR, gDNA) With Recovery Beads

Description:

DNA Normalization Magnetic Beads (with recovery beads) were developed for the normalization of NGS libraries, PCR fragments, sheared genomic DNA, and genomic DNA based on the magnetic bead technology from the American company BioDynami. These magnetic beads have DNA-binding capability, and due to the limited DNA-binding capacity, a normalized amount of DNA is recovered while excess unbound DNA is separated.

A normalized DNA concentration can be obtained from DNA samples of varying concentrations. Unlike traditional fluorescent DNA quantification methods, this protocol is simple, fast, centrifugation-free, and filtration-free. After DNA normalization with magnetic beads, no further DNA quantification or dilution is required.

Efficiency:

The graphs illustrate the effective DNA normalization for NGS libraries and PCR fragments using magnetic beads. In both cases, the first graph compares a wide range of DNA input amounts (from 300 ng to 2000 ng) with the resulting normalized amount. The second graph provides a more detailed view, showing that regardless of the input DNA concentration, the magnetic beads lead to a consistent output. The normalized DNA amount falls within a relatively narrow range, approximately between 100 ng and 180 ng, ensuring reproducible and reliable results for downstream applications while eliminating the need for further quantification and concentration adjustments. This uniformity is key for optimizing and streamlining molecular biology workflows.

The third graph demonstrates genomic DNA normalization using magnetic beads. Again, despite a broad range of DNA input amounts (from 500 ng to 5000 ng), a relatively consistent output is achieved. The normalized DNA amount ranges approximately from 220 ng to 270 ng. This confirms that the magnetic bead technology effectively normalizes genomic DNA with varying input concentrations, ensuring reliable and comparable results in subsequent experiments.

Specifications:

Features:

• Consistent normalization from various sources: NGS libraries, PCR fragments, and genomic DNA
• Recovery of excess unbound DNA (Cat. No. 40062S & 40062L)
• Excellent dynamic range
• NGS Library, PCR, sheared genomic DNA: 7-fold difference in input DNA
• Genomic DNA: 10-fold difference in input DNA
• Simple and fast protocol yielding double-stranded DNA
• No fluorescent quantification required
• No centrifugation, no filtration

Applications:

• Normalization of NGS libraries
• Target enrichment
• PCR normalization
• Genomic DNA and sheared genomic DNA normalization

Package Sizes:

48 reactions (Cat. No. 40072S)
96 reactions (Cat. No. 40072L)