VWR®, Thermal Cyclers Doppio Gradient

Description:
The VWR® Thermal Cycler Doppio Gradient is a professional gradient PCR thermal cycler equipped with two independent 48-well gradient blocks in one system. It combines maximum flexibility, efficient laboratory space utilization, and the ability to run two independent PCR experiments with different temperature conditions simultaneously.

Each block operates independently and is compatible with 48 × 0.2 ml PCR tubes or 24 × 0.5 ml flat-cap tubes. Gradient technology enables PCR optimization by testing different temperature conditions within a single run, helping determine optimal primer annealing temperatures and develop new PCR protocols.

The system uses powerful Peltier elements with Long Life Technology, providing rapid temperature changes up to 5 °C/s and excellent block temperature uniformity. Remote control, protocol management, and monitoring are available through VWR PCR Cycler Master Software.

Features:

• two independent high-speed 48-well gradient PCR blocks in one system
• simultaneous operation of two separate PCR programs
• each block compatible with:
  • 48 × 0.2 ml PCR tubes
  • 24 × 0.5 ml flat-cap tubes
• gradient technology for PCR optimization
• linear temperature gradient capability
• independent lane control
• Long Life Technology Peltier elements
• temperature change rate up to 5 °C/s
• temperature control accuracy ±0.1 °C
• block homogeneity ±0.2 °C at 72 °C
• touch-sensitive TFT display (800 × 480 pixels, 16:9, 65,536 colours)
• automatically adjustable heated lid height
• remote control and monitoring via PCR Cycler Master Software
• PC-based PCR protocol creation
• emulation mode for easy PCR program transfer
• Global Program Ramp, Gradient Control and Tube Control functions
• internal memory for up to 500,000 PCR protocols
• 4× USB and 1× Ethernet interfaces
• compatible with Windows and Linux systems
• manufactured in Germany according to ISO 9001
• calibrated and maintained according to NIST standards

Applications:

• PCR optimization
• primer annealing temperature optimization
• standard PCR
• DNA amplification
• molecular biology
• genetic analysis
• research laboratories
• parallel PCR workflows