One-Step RT-PCR Kit

Description:

The One-Step RT-PCR Kit combines reverse transcription and PCR in a single reaction, eliminating the need for two-step protocols. This approach avoids the transfer of reaction mixtures between the reverse transcription and PCR steps, reducing the risk of contamination and minimizing labor-intensive sample handling.

The unique buffer and enzyme mix formulation ensures reliable and consistent results. The optimized buffer composition enables efficient reverse transcription and PCR even when working with RNA containing secondary structures, ensuring complete cDNA synthesis and high specificity of PCR fragments.

The buffer in the One-Step RT-PCR Kit contains dNTPs, MgCl₂, a PCR enhancer, and a PCR stabilizer. The enzyme mix includes M-MuLV reverse transcriptase, Taq DNA polymerase, an enzyme enhancer, and a stabilizer.
M-MuLV reverse transcriptase is an RNA-dependent DNA polymerase that synthesizes DNA strands using RNA as a template. The reverse transcriptase is derived from a cloned M-MuLV RT gene.
Taq DNA polymerase is a thermostable DNA polymerase with 5′→3′ polymerase activity and low 5′→3′ exonuclease activity. It is derived from a cloned gene of Thermus aquaticus YT-1.

The image shows the electrophoresis results of PCR products obtained using the One-Step RT-PCR Kit. Clearly visible bands correspond to the expected sizes of target DNA fragments, confirming successful and specific amplification. Size markers confirm accurate product sizes for both small and large fragments. These results demonstrate the reliability, precision, and high performance of the kit for amplifying both RNA and DNA templates.

Features:

• cDNA synthesis and PCR combined in a single step

• Robust and reliable reactions

• Unique buffer and enzyme formulation delivers consistent results

• PCR fragments feature 3'-dA overhangs

Package sizes:

100 reactions (Cat. No. 50503S)
500 reactions (Cat. No. 50503L)