One-Step RT-PCR Kit (Probe-Based)

Description:

The One-Step RT-qPCR Kit (Probe-Based) is a highly sensitive and specific real-time PCR kit that utilizes fluorescent probes. This kit enables one-step detection and quantification of RNA—both reverse transcription and subsequent PCR amplification are performed in a single tube, saving time and reducing the risk of contamination.

A specially optimized mix of enzymes and buffers ensures rapid and efficient cDNA synthesis as well as amplification of the target sequence. High specificity is achieved through the use of fluorescent probes that recognize only the target sequence, thereby minimizing the formation of nonspecific products. The kit is compatible with most real-time PCR instruments thanks to the included ROX reference dye for signal normalization.

This product is suitable for a wide range of applications—from screening to validation—and delivers consistent, reproducible results even with very low sample concentrations.

The graph confirms the high sensitivity and efficiency of the Probe-Based One-Step RT-qPCR Kit. All samples show clear and specific amplification with no signs of inhibition or nonspecific products. The kit enables reliable quantification of even low concentrations of target sequences, making it ideal for accurate and reproducible results in molecular diagnostics.

Features:

• Fast and easy setup – all in one reaction
• High sensitivity and specificity – detects even very low amounts of RNA
• Minimizes contamination risk – no step-to-step transfers
• Universal compatibility – suitable for all common real-time PCR instruments
• Consistent and reproducible results – ideal for screening and validation

Package sizes:

100 reactions (Cat. No. 50504PS)
500 reactions (Cat. No. 50504PL)