MagnifiQ™ 96 Genomic DNA instant kit

Description:
MagnifiQ 96 Genomic DNA instant kit is designed for automated magnetic isolation of DNA from various types of biological material in a 96-samples-per-plate format. It contains ready-to-use, reagent-filled plates and all necessary consumables. The kit allows isolation of 96 samples in a single extraction run. The MagnifiQ product series is based on automated nucleic acid isolation using magnetic beads. According to the manufacturer, this approach significantly shortens working time and reduces the risk of error compared to manual methods. The isolated material is, according to the manufacturer, suitable for downstream analysis by qPCR and RT-PCR as well as for sequencing. The manufacturer also states that no initial preparation of buffers is required: it is enough to add samples to the plate and the extracted material is available in approximately half an hour. According to the manufacturer, different sample types can be processed with one universal kit and an automated extraction programme.

Features:

• Automated magnetic isolation of DNA using magnetic beads
• 96 samples per plate format
• Plates pre-filled with reagents
• No buffer preparation required
• Protocol time approx. 30 min
• Elution volume 100 µl
• Elution solution: Tris buffer
• Binding capacity 30 µg DNA
• Universal use for bacteria (G−, G+), yeast, cell cultures, blood, serum, plasma, animal tissue, swabs and feces
• Tested with ThermoFisher Scientific KingFisher Flex and Allsheng Auto-Pure 96 devices. According to the manufacturer, this does not preclude use with other devices
• Optional RNA removal with RNase (not included)
• For research use only

Applications:

• Bacterial cultures (G−, G+): the separately supplied Bacteria lysis kit is required for sample preparation
• Yeast cultures: the separately supplied Yeast lysis kit is required for sample preparation
• Cell cultures
• Fresh or frozen blood, serum and plasma
• Animal tissue
• Swabs: swabs in transport medium require no additional preparation, while for dry swabs the part of the swab with the sample is lysed directly in the wells
• Feces and environmental samples (soil, activated sediment, compost), including samples stored in conservation solution: the separately supplied Microbiome lysis kit is required for sample preparation, or the StoolSave DNA Protection kit for samples stored in it
• DNA preparation for qPCR, RT-qPCR and sequencing

Kit contents:

• CP comb plate
• SP-D sample plate
• WP 1 and WP 2-3 wash plates
• EP elution plate
• Proteinase K
• LTE 2X buffer
• Tris buffer
• LSDE buffer
• Tip comb 96
• Protective film

Specification:

Protocol files: