MagnifiQ™ 1 Total RNA Plus instant kit

Description:
MagnifiQ 1 Total RNA Plus instant kit is designed for automated magnetic isolation of total RNA from various types of biological material in a strip format. It contains ready-to-use, reagent-filled strips and all necessary consumables. The strip format enables RNA isolation from a single sample per purification run. The MagnifiQ product series is based on automated nucleic acid isolation using magnetic beads. According to the manufacturer, this approach significantly shortens working time and reduces the risk of error compared to manual methods. The isolated material is, according to the manufacturer, suitable for downstream analysis by RT-PCR methods as well as for sequencing. The manufacturer also states that only a few minutes of manual work are needed for adding the samples, with the rest of the procedure carried out by the automatic extraction device, and that no initial preparation of buffers is required: the extracted material is available after about 30 minutes. According to the manufacturer, different sample types can be processed with one universal kit and an automated extraction programme.

Features:

• Automated magnetic isolation of total RNA using magnetic beads
• XS-R strips pre-filled with reagents
• RNA isolation from a single sample per run
• No buffer preparation required
• Protocol time approx. 30 min
• Elution volume 100 µl
• Elution solution: Tris buffer
• Binding capacity 30 µg RNA
• Universal use for bacteria (G−, G+), cell cultures, fresh blood, blood in PAXgene Blood RNA Tubes and solid tissue
• Fenozol Plus lysis reagent included
• Tested with Allsheng Auto-Pure Mini and Auto-Pure S32 devices. According to the manufacturer, this does not preclude use with other devices
• For research use only

Applications:

• Gram-positive (G+) bacteria: the separately supplied BacBreaker enzyme mix is required for sample preparation, and lysostaphin for staphylococci
• Gram-negative (G−) bacteria
• Cell cultures
• Fresh (not frozen) blood: erythrocytes are lysed with RBCL solution before isolation (not included)
• Blood collected in PAXgene Blood RNA Tubes
• Solid tissue: tissue homogenized in liquid nitrogen, with mechanical lysis using zirconia beads for soft tissues
• RNA preparation for reverse transcription, RT-qPCR and transcriptome sequencing

Kit contents:

• XS-R extraction strips
• Fenozol Plus (storage at 2–8 °C)
• Ultrapure water
• Tip comb 8

Specification:

Protocol files: