MagnifiQ™ Genomic DNA reagents and consumables kit

Description:
MagnifiQ Genomic DNA reagents and consumables kit is a set of reagents and all necessary consumables for filling plates for the automated, magnetic isolation of DNA from various types of biological material. The user fills the plates with reagents following the diagram in the manual, in either a 16-samples-per-plate or a 96-samples-per-plate format. The MagnifiQ product series is based on automated nucleic acid isolation using magnetic beads. According to the manufacturer, this approach significantly shortens working time and reduces the risk of error compared to manual methods. The isolated material is, according to the manufacturer, suitable for downstream analysis by qPCR and RT-PCR as well as for sequencing. The manufacturer also states automated, fast isolation and the ability to process different samples with one universal kit and an automated extraction programme.

Features:

• Set of reagents and consumables for filling plates for automated magnetic DNA isolation
• 16 samples per plate and 96 samples per plate formats (depending on the kit variant)
• Protocol time approx. 30 min
• Elution volume 50–100 µl
• Elution solution: Tris buffer
• Binding capacity 30 µg DNA
• Universal use for bacteria (G−, G+), yeast, cell cultures, blood, serum, plasma, animal tissue, swabs and feces
• Tested with Allsheng Auto-Pure Mini, Auto-Pure 32A and Auto-Pure 96 devices. According to the manufacturer, this does not preclude use with other devices
• 80% ethanol, an automated pipette and pipette tips are required for plate preparation (not included)
• Optional RNA removal with RNase (not included)
• For research use only

Applications:

• Bacterial cultures (G−, G+): the separately supplied Bacteria lysis kit is required for sample preparation
• Yeast cultures: the separately supplied Yeast lysis kit is required for sample preparation
• Cell cultures
• Fresh or frozen blood, serum and plasma
• Animal tissue
• Swabs: swabs in transport medium require no additional preparation, while for dry swabs the part of the swab with the sample is lysed directly in a tube or in the wells
• Feces and environmental samples (soil, activated sediment, compost), including samples stored in conservation solution: the separately supplied Microbiome lysis kit is required for sample preparation, or the StoolSave DNA Protection kit for samples stored in it
• DNA preparation for qPCR, RT-qPCR and sequencing

Kit contents:

• MQBG binding mix
• A1WI wash solution
• Tris buffer
• LSDE buffer
• LTE 2X buffer
• Proteinase K
• 2.2 ml plates
• 0.5 ml plates (96-sample variant)
• CP comb plate (96-sample variant)
• Tip comb 8 (16-sample variants) or tip comb 96 (96-sample variant)
• Protective film

Specification:

Protocol files: