CHAPS

TE Buffer is a common molecular biology buffer used for protection of DNA and RNA from degradation. The Tris buffering agent and EDTA metal chelating properties help to protect DNA and RNA.

TAE buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis¹. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. 

Ammonium acetate is a widely used reagent in molecular biology and chromatography. Suitable applications include the purification and precipitation of DNA and protein crystallization. Ammonium acetate is commonly used in HPLC and MS analysis of various compounds, such as oligosaccharides, proteins, and peptides.

D-(+)-Glucose is a common natural sugar involved in processes such as energy production, glycosylation, and formation of glycans that provide structure to cells. Glucose is involved in a detrimental process in cells called glycation. Glucose is used as a supplement for cell culture and in numerous cellular processes and molecular biology applications.

Kanamycin Sulfate solution is an aminoglycoside antibiotic consisting of a six-membered aminocyclitol ring attached to aminosugar side chains through glycosidic bonds. Kanamycin Sulfate solution acts by inhibiting protein synthesis, and compromising translational fidelity in susceptible bacteria.

Brij-35 (30% Solution) is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or as a surfactant in various HPLC applications.

The loading dye increases the density of the sample and adds colours to the sample, thereby simplifying the loading process. The solution contains dyes that, in a electric field, move toward the anode at predictable rates.

LB Agar plates contain 1.0% Tryptone, 0.5% yeast extract, 0.5% NaCl, 1.5% agar.

A routine use of agarose is ideal for daily analysis of nucleic acids by gel electrophoresis or blotting (Northern or Southern) and is also suitable for protein applications such as Ouchterlony and radial immunodiffusion (RID). It has low ethidium bromide and SYBR Green background staining.

HEPES Solution is a biological buffer useful in cell culture media. Selection of suitable nutrient medium is dependent on type of cell, conditions of culture, and degree of chemical definition required for the cell culture application.

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation.

MCLAB's RNA Storage Buffer, with optimized reagents and pH value, provides better preservation and greater RNA stability for valuable RNA samples than the standard EDTA and TE Buffer or just water.

Tris Buffered Saline (TBS) is an isotonic, non-toxic buffer used in some biomedical techniques to maintain pH within a relatively narrow range. TBS can shift pH relatively drastically as temperature changes, with the pKa declining approximately 0.03 units per degree Celsius rise in temperature.

5M solution of sodium chloride (NaCl) in high purity dH₂O. Solution is 0.2 μm filtered and dispensed into durable, square Nalgene bottles.