DEPC-Treated Water
DEPC-treated Water is deionized, high quality, molecular biology grade water.
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CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins.
Description
CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins, which are often sparingly soluble or insoluble in aqueous solution due to their native hydrophobicity. CHAPS can also be used in conjunction with nonionic detergents such as Triton X-100.
Applications
Solublizies proteins in non-denaturing state
Helps monitor membrane proteins due to its low background absorbance in the UV region
Synonyms
3-[(3-Chloromidopropyl)dimethylammonio]-1-propanesulfonate
Molecular Formula
C32H58N2O7S
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DEPC-treated Water is deionized, high quality, molecular biology grade water.
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CHAPS (3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate ) is a zwitterionic detergent used to solubilize proteins. It is used as a non-denaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins.
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