microRNA & Oligo Purification Beads

Description:

BioDynami's magnetic beads for microRNA and oligonucleotide purification are designed to address the limitations of Solid Phase Reversible Immobilization (SPRI) technology, which struggles to efficiently capture DNA/RNA fragments shorter than 100 bases. Thanks to their unique technology, BioDynami magnetic beads overcome the low recovery efficiency of short DNA/RNA fragments. These beads are ideal for purifying microRNA, oligonucleotides, and short DNA/RNA fragments, while effectively removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants. The reagents for the magnetic beads are RNase-free, making them suitable for both DNA and RNA applications.

The magnetic beads are optimized for purifying microRNA, oligonucleotides, and DNA/RNA fragments. Fragments can be as short as 20 bases, including microRNA, tRNA, dsDNA (20 bp and longer), ssDNA (20 nt and longer), RNA (20 nt and longer), DNA/RNA hybrids (20 bp and longer), and oligonucleotides (20 nt and longer).

The comparison of the efficiency of different types of magnetic beads for purifying DNA fragments of various lengths is shown in the gel electrophoresis image. The columns display the purification results using AMPure® XP*, BioDynami Magnetic Beads (DNA & RNA), and BioDynami Magnetic Beads (microRNA & Oligo). The graph clearly shows that BioDynami Magnetic Beads (microRNA & Oligo) effectively capture short DNA fragments of 20–100 bp, whereas AMPure® XP* beads are unable to purify fragments shorter than 100 bp.

Procedure:

The DNA/RNA purification procedure using magnetic beads is shown in the diagram. It includes adding beads to the sample, where the DNA/RNA binds to their surface. A magnet is then used to separate the beads with the bound DNA/RNA from the impurities. The beads are washed to remove any remaining contaminants, and finally, the purified DNA/RNA is eluted into a new solution for further use.

Efficiency:

As shown in the graph, the highest recovery rate is achieved for DNA (20 bp), reaching nearly 90%, while DNA and RNA oligonucleotides show similar results around 85%. This confirms that BioDynami's magnetic beads for microRNA and oligo purification are highly efficient in isolating short fragments, which is crucial for applications requiring clean and high-quality nucleic acids.

Features:

Efficient cleaning of short DNA and RNA samples:

• MicroRNA
• tRNA
• dsDNA fragments 20 bp or longer
• ssDNA fragments 20 nt or longer
• RNA fragments 20 nt or longer
• DNA/RNA hybrid fragments 20 bp or longer
• Oligo and chimeric oligos 20 nt or longer

Removal of impurities and unwanted reaction components
Compatibility: Works with both manual and automated procedures

Package Sizes:

• 50 reactions (cat. no. 40052S)
• 200 reactions (cat. no. 40052M)

* AMPure® XP and SPRIselect are trade marks of Beckman Coulter.