Thidiazuron (TDZ) Powder

Description:

Thidiazuron (TDZ) is a plant growth regulator and a synthetic diphenylurea cytokinin, widely used in plant tissue culture to promote shoot regeneration, organogenesis, and somatic embryogenesis.

Features:

• Easy to use
• Effective stimulation of shoot multiplication from explants (micropropagation)
• Used as an additive in tissue culture media, e.g., MS medium
• Helps overcome apical dominance
• Essential for micropropagation of certain plant species

Preparation guide:

• Prepare liquid plant culture medium using MS or DKW medium depending on the plant type.
• Add sugar (typically 30 g per 1 liter of medium), 1 ml/l PPM, and 3–4 g/l gellan gum or 6–8 g/l agar.
• Add the required amount of plant hormone.
• Autoclave the medium for 20 minutes at 15 psi and 121 °C.

Usage:

• For standalone use or in combination with other hormones for efficient in vitro plant multiplication.
• Seed germination stimulation in culture media.
• Suitable for use in agriculture as well as home gardening.
• Commonly used together with auxins (e.g., IAA) to achieve a balance between shoot and root development in the medium.

Video tutorial:

How To Make Stock Solutions For Plant Growth Regulators
How To Use Plant Growth Regulators For Plant Tissue Culture

Package size:

1 g