MagnifiQ™ 16 ExTerminator instant kit

Description:
MagnifiQ 16 ExTerminator instant kit is designed for automated magnetic removal of nucleotide dye terminators from cycle sequencing reaction products in a 16-samples-per-plate format. It contains ready-to-use, reagent-filled plates and all necessary consumables. The kit is intended for rapid and reliable cleanup before capillary electrophoresis sequencing. According to the manufacturer, the purified material is suitable for analysis on automated DNA sequencers including ABI3100, SeqStudio and other genetic analysers capable of resolving BigDye terminator chemistries. Like previous ExTerminator kits from A&A Biotechnology, this product also provides visual control of the purification process by means of Mix Blue reagent. According to the manufacturer, no initial preparation of buffers is required, only minutes of manual work are needed, and the purified products are ready for sequencing after approximately 23 minutes.

Features:

• Automated magnetic removal of dye terminators after cycle sequencing reactions using magnetic beads
• 16 samples per plate format
• XP-T plates pre-filled with reagents
• No buffer preparation required
• Protocol time approx. 23 min
• Sample size up to 20 µl of cycle sequencing products
• Elution volume 50 µl
• Elution solution: ultrapure water, optionally deionised formamide (not included)
• Binding capacity 10 µg DNA
• Visual control of purification with Mix Blue reagent: light blue color of the eluates confirms a correct purification process
• Purified products can be loaded directly onto a genetic analyser (SeqStudio, 310, 3100, 3100-Avant, 3130 and 3130xl)
• Tested with ThermoFisher Scientific KingFisher Flex and Allsheng Auto-Pure Mini, Auto-Pure S32 and Auto-Pure 32A devices. According to the manufacturer, this does not preclude use with other devices
• For research use only

Applications:

• Removal of nucleotide dye terminators from cycle sequencing reaction products before capillary electrophoresis sequencing
• Sample preparation for capillary electrophoresis: de novo Sanger DNA sequencing, plasmid sequencing, microbial species identification, single-nucleotide polymorphism (SNP) genotyping and CRISPR-Cas9 genome editing analysis

Kit contents:

• XP-T extraction plates
• Mix Blue
• Tip comb 8
• Protective film

Specification:

Protocol files: