MagnifiQ™ 16 Plant DNA instant kit

€214.50
Tax excluded
Plant DNA isolation
16-sample format
Dry and fresh tissue
Quantity: 64 isolations
€214.50
Tax excluded
Quantity

Description:
MagnifiQ 16 Plant DNA instant kit is designed for automated magnetic isolation of genomic DNA from plant tissue in a 16-samples-per-plate format. It contains ready-to-use, reagent-filled plates and all necessary consumables. The MagnifiQ product series is based on automated nucleic acid isolation using magnetic beads. According to the manufacturer, this approach significantly shortens working time and reduces the risk of error compared to manual methods. The isolated material is, according to the manufacturer, suitable for downstream analysis by PCR and real-time PCR as well as for sequencing. The manufacturer also states that no initial preparation of buffers is required: it is enough to add prepared samples to the plate and the extracted material is available in approximately half an hour. According to the manufacturer, different sample types can be processed with one universal kit and an automated extraction programme.

Features:

  • Automated magnetic isolation of genomic DNA from plant tissue using magnetic beads
  • 16 samples per plate format
  • XP-PT plates pre-filled with reagents
  • No buffer preparation required for the isolation itself
  • Protocol time approx. 31 min
  • Elution volume 100 µl
  • Elution solution: Tris buffer
  • Binding capacity up to 60 µg
  • Suitable samples: dry, or fresh or frozen plant tissue, either powdered or unpowdered
  • Tested with Allsheng Auto-Pure Mini, Auto-Pure S32 and Auto-Pure 32A devices. According to the manufacturer, this does not preclude use with other devices
  • Optional RNA removal with RNase (not included)
  • For research use only

Applications:

  • Genomic DNA isolation from dry plant tissue: powdered or unpowdered
  • Genomic DNA isolation from fresh or frozen plant tissue: powdered or unpowdered. Unpowdered tissue is homogenized before lysis in M45 tubes with metallic beads, which are a separate product and are not included
  • DNA preparation for qPCR, RT-PCR and sequencing

Kit contents:

  • XP-PT extraction plates
  • LPE lysis buffer
  • Proteinase K (storage at 2–8 °C)
  • L3P precipitation solution
  • Tip comb 8
  • Protective film

Specification:

Parameter Value
Protocol time approx. 31 min
Elution volume 100 µl
Elution solution Tris buffer
Binding capacity up to 60 µg
Downstream applications   qPCR, RT-PCR, sequencing
Sample type Sample size
Dry, powdered plant tissue 20–50 mg
Fresh or frozen, powdered plant tissue 40–80 mg
Dry, unpowdered plant tissue 20–50 mg
Fresh or frozen, unpowdered plant tissue   40–80 mg

Protocol files:

Device Protocol name Installation
Auto-Pure Mini MQ-PLT-MI 1. Create a folder “items” on a USB drive and copy the protocol file to it.
2. Insert the USB drive into a USB slot on the device.
3. On the device screen, go to Settings > System > Transfer > Import.
4. Select the protocol and tap “Import”.
Auto-Pure Mini    MQ-PLT-MI 1. On the device screen, go to Run.
2. Scan the QR code with the device’s scanner.
Auto-Pure 32A MQ-PLT-32A 1. Create a folder “items” on a USB drive and copy the protocol file to it.
2. Insert the USB drive into a USB slot on the device.
3. On the device screen, go to Settings > Im.&Export > Import.
4. Select the protocol and tap “Import”.
Auto-Pure S32 MQ_PLT_S32 1. Create a folder “im_export_protocols” on a USB drive and copy the protocol file to it.
2. Insert the USB drive into a USB slot on the device.
3. On the device screen, go to Protocols > Import.
4. Select the protocol and tap “Import”.
650A-16V-64
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