Recombinant Human Epiregulin Protein

Description
Epiregulin is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration.  Epiregulin is expressed primarily in the placenta and macrophages. High level expression has also been detected in various carcinomas. Epiregulin specifically binds EGFR (ErbB1) and ErbB4 but not ErbB2 and ErbB3. It activates the homodimers of both ErbB1 and ErbB4. In addition, epiregulin can also activate all possible heteromeric combinations of the four ErbB family members. Epiregulin stimulates the proliferation of fibroblasts, smooth muscle cells and hepatocytes. It has been shown to be an autocrine growth factor for epidermal keratinocytes as well as mesangial cells. Epiregulin has also been shown to inhibit growth of several epithelial tumor cells. In addition, Epiregulin has been implicated in the implantation process during pregnancy.

Full Name
Epiregulin

Source
E. coli

Species
Human

Accession #
O14944

Molecule Weight
The mature recombinant human Epiregulin is a 5.4 kDa protein containing 46aa (V63-L108).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.125-0.75 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS