Recombinant Human Epigen (EPGN) Protein

Description
Epigen (EPGN) is a member of the epidermal growth factor family. Members of this family are ligands for the epidermal growth factor receptor and play a role in cell survival, proliferation and migration.  Epign is expressed in a variety of tissues, including hepatic, testicular, cardiac, pulmonary, and others. Upon binding to EGF receptor expressed on epithelial cells, epigen triggers mitosis. The strong mitogenic potential can be explained by the low-affinity binding to EGF receptor, which results in downregulation of receptor ubiquitination. Epigen is also expressed in adenocarcinomas, suggesting a role in oncogenesis.

Full Name
Epigen

Source
E. coli

Species
Human

Accession #
Q6UW88

Molecule Weight
The mature recombinant human EPGN is a 6 kDa protein containing 51aa (L54-A104).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 100-500 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS