Recombinant Human FGF-6 Protein

Description
Fibroblast growth factor 6 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. 
FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect. FGF-6 expression is restricted to skeletal muscle during development, although it can be upregulated in prostate cancer and Kaposi sarcoma.

Full Name
Human Fibroblast Growth Factor 6

Source
E. coli

Species
Human

Accession #
P10767

Molecule Weight
The mature recombinant human FGF-6 consists of 168 amino acids (41-208) and has a predicted molecular mass of 18.7 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using NR6R­3T3 mouse fibroblast cells. The ED50 for this effect is 0.1-0.3 ng/mL in the presence of 1 µg/mL of heparin.

 Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS