Recombinant Human INF-gamma Protein

Description
The interferons (IFN)s are a family of cytokines with potent antiviral, antitumor, and immunomodulatory properties. Interferons are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression.
IFN- γ (interferon gamma) is a potent multifunctional cytokine, which is secreted primarily by activated NK cells and T cells. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Full Name
Interferon gamma

Source
E. coli

Species
Human

Accession #
NM_000619

Molecule Weight
The mature recombinant human IFN- γ is a 17 kDa protein (24-166aa).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.15-0.75 ng/mL.
The specific activity of Recombinant Human IFN-gamma is approximately 2 x 104 IU/μg, which is calibrated against human IFN-gamma Standard (NIBSC code: 87/586).

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS