Recombinant Human INF-epsilon Protein

Description
The interferons (IFN)s are a family of cytokines with potent antiviral, antitumor, and immunomodulatory properties. Interferons are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression.
IFN- ε (interferon epsilon) signals through IFN‑alpha / beta R1 and IFN‑alpha / beta R2 and contributes to TNF-alpha induced signaling. It is constitutively expressed in epithelial cells lining the lung, intestines, testes, and female reproductive tract, and it is further up-regulated in the uterus by estrogen. IFN-epsilon provides mucosal barrier protection against the pathogens Chlamidia and Herpes simplex virus 2. It induces B cell activation, attraction of CD8+ T cells to sites of virus infection and directly mediates protection against viral and bacterial genital infections. Differential regulation of genes related to central nervous system by recombinant human IFN-epsilon suggests its role in maintenance of the structure and function of brain.

 Full Name
Interferon epsilon

Source
E. coli

Species
Human

Accession #
Q86WN2

Molecule Weight
The mature recombinant human IFN- ε is a 23 kDa protein (22-208aa).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 100-500 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS