Recombinant Human INF-kappa Protein

Description
The interferons (IFN)s are a family of cytokines with potent antiviral, antitumor, and immunomodulatory properties. Interferons are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. Interferons are divided into type I, II, and III. Type I IFNs (IFN-α and IFN-β) are most abundant in number, distribution, and expression.
IFN- κ (interferon kappa) is a 24 kDa secreted, nonglycosylated polypeptide that belongs to the IFN-κ subclass of the type I interferon family of cytokines. It is expressed by macrophages in mice, and monocytes, dendritic cells and keratinocytes in human. IFN-κ induces TNF-α, IL-10 and MCP-1 production by monocytes.

 Full Name
Interferon kappa

Source
E. coli

Species
Human

Accession #
Q9P0W0

Molecule Weight
The mature recombinant human IFN-κ is a 21 kDa protein (22-199aa).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 0.2-1.2 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS