Recombinant Human Neurturin Protein

Description
Neurturin is a disulfide-linked homodimer neurotrophic factor structurally related to GDNF, artemin, and persephin.  These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures.  Neurturin signals through a multicomponent receptor system, composed of RET and one of four GFRα (α1-α4) receptors.  Neurturin promotes the development and survival of sympathetic and sensory neurons by signaling through a receptor system composed of RET and GFRα2. 
The functional form of human neurturin is a disulfide-linked homodimer, of two 11.8 kDa polypeptide monomers (204 total amino acid residues.

Full Name
Human Neurturin protein

Source
E. coli

Species
Human

Accession #
Q99748

Molecule Weight
The mature recombinant human Neurturin protein consists of 102 amino acids (96-197) and has a predicted molecular mass of 23.6 kDa as a dimer.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is typically 20-80 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS