Recombinant Human TGF-beta 1 Protein

Description
TGF-beta 1 (transforming growth factor beta 1) is one of three closely related mammalian members, TGF-beta 1, -2 and -3 , of the large TGF-beta superfamily that share a characteristic cystine knot structure. These three TGF-beta isoforms are highly pleiotropic cytokines that are proposed to act as cellular switches that regulate processes such as immune function, proliferation and epithelial-mesenchymal transition. They are involved in various physiological processes, including embryogenesis, tissue remodeling and wound healing. They are secreted predominantly as latent complexes, which are stored at the cell surface and in the extracellular matrix. 
TGF-beta1 is the most abundant isoform secreted by almost every cell type. It was originally identified for its ability to induce phenotypic transformation of fibroblasts, and recently it has been implicated in the formation of skin tumors.

Full Name
Transforming growth factor beta 1

Source
E. coli
Species
Human

Accession #
P01137

Molecule Weight
The mature recombinant human TGF-beta1 consists of 112 amino acids (279-390) and has a predicted molecular mass of 13 kDa.

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to inhibit the IL-4-dependent proliferation of HT‑2 mouse T cells. The ED50 for this effect is 0.04-0.2 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS