Recombinant Human VEGF Protein

Description
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a potent polypeptide regulator of blood vessel function. VEGF promotes an array of responses in the endothelium, including hyperpermeability, endothelial cell growth, angiogenesis, and enhanced glucose transport. It is a member of the PDGF family that is characterized by the presence of eight conserved cysteine residues and a cystine knot structure.

Full Name
Vascular endothelial growth factor

Source
E. coli

Species
Human

Accession #
NP_001165097

Molecule Weight
The mature recombinant human VEGF is a 19.2 kDa protein (27-191aa).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 1-6 ng/mL. The specific activity of recombinant human VEGF is approximately 1.7 x 103 U/μg, which is calibrated against recombinant human VEGF WHO Standard.
Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS