Recombinant Human Wnt-5b Protein

Description
The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Wnt family members are highly hydrophobic, and possess 22 to 24 conserved cysteines.
Human Wnt‑5b has 342 aa in the mature region. Although Wnt‑5a and Wnt‑5b share 83% aa identity, their effects may be different. For example, Wnt-5b, but not Wnt‑5a, promotes cell cycle progression and is weakly transforming. Wnt‑5a and ‑5b are thought to coordinate developmental events, such as chondrocyte differentiation and formation of endochondral bone. Wnt‑5b is up‑regulated during early adipogenesis, and its overexpression in 3T3‑L1 cells partially inhibits canonical Wnt suppression of adipogenesis. It enhances PPAR gamma expression and promotes differentiation of preadipocytes. Human Wnt‑5b polymorphisms have been associated with Type II diabetes.

Full Name
Wingless-type MMTV Integration Site Family Member 5b

 Source
E. coli

Species
Human

Accession #
NP_110402

Molecule Weight
The mature recombinant human Wnt-5b is a 38.5 kDa protein containing 342aa (Gln18-Lys359).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 125-750 ng/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS