Recombinant Human Wnt-7a Protein

Description
The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Wnt family members are highly hydrophobic, and possess 22 to 24 conserved cysteines.
Human Wnt‑7a is expressed by epithelial and epithelially-derived cells of the placenta, kidney, testis, uterus, fetal lung and fetal and adult brain. Wnt-7a is frequently downregulated in leukemia and lung cancers, potentially affecting homeobox (HOX) gene expression, differentiation state and growth control. Roles for Wnt-7a have also been shown during formation of neural synapses, response of the uterus to estrogen and inflammatory cartilage destruction. 

Full Name
Wingless-type MMTV Integration Site Family Member 7a

 Source
E. coli

Species
Human

Accession #
O00755

Molecule Weight
The mature recombinant human Wnt-7a is a 35.5 kDa protein containing 318aa (L32-K349).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 1-3 µg/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a. 

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS