Recombinant Human Wnt-10b Protein

Description
The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Wnt family members are highly hydrophobic, and possess 22 to 24 conserved cysteines.
Wnt‑10b plays a critical role in maintaining mesenchymal stem cells and determining whether they differentiate to adipocytes or osteoblasts. Wnt-10b is mainly produced by stem cells and pre-osteoblasts, but also by adult bone marrow CD8+ T lymphocytes stimulated with parathyroid hormone. In some hepatocellular carcinomas, Wnt-10b can inhibit cancer cell growth, but in others, it can act synergistically with FGFs to stimulate cell growth. Several Wnts, including Wnt-10b, are expressed in both normal and/or malignant colon tissues. 

Full Name
Wingless-type MMTV Integration Site Family Member 10b

 Source
E. coli

Species
Human

Accession #
NP_003385

Molecule Weight
The mature recombinant human Wnt-10b is a 40 kDa protein containing 389aa (M1-K389).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.3-1.2 μg/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS