Recombinant Human Wnt-11 Protein

Description
The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Wnt family members are highly hydrophobic, and possess 22 to 24 conserved cysteines.
Wnt‑11 is required for cardiogenesis, organization of early muscle fibers, and branching morphogenesis of ureters, expressed in early development during gastrulation and in the perichondrium, lung mesenchyme and ureteric buds, but not in neuroepithelium. Wnt‑11 often, but not exclusively, signals by a non‑canonical pathway and may repress canonical signaling by other Wnts.

Full Name
Wingless-type MMTV Integration Site Family Member 11

 Source
E. coli

Species
Human

Accession #
NP_004617

Molecule Weight
The mature recombinant human Wnt-11 is a 36.6 kDa protein containing 330aa (I25-K354).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation/survival assay using IEC‑18 rat small intestinal epithelial cells. The ED50 for this effect is 0.6-2.4 μg/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS