Recombinant Human Wnt-16b Protein

Description
The Wnts is a large family of secreted proteins with roles in pattern formation, cell fate decision, axon guidance, and tumor formation. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self-renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Wnt family members are highly hydrophobic, and possess 22 to 24 conserved cysteines.
Wnt‑16a and Wnt‑16b isoforms in humans differ in the signal sequence and the first two amino acids (aa) of the mature protein. Wnt‑16b is the more conserved isoform and is widely expressed. Wnt‑16 signaling through both canonical and JNK‑mediated (non‑canonical) pathways is reported.

Full Name
Wingless-type MMTV Integration Site Family Member 16b

 Source
E. coli

Species
Human

Accession #
Q9UBV4

Molecule Weight
The mature recombinant human Wnt-16b is a 37.6 kDa protein containing 336aa (N30-K365).

Purity
> 95 % as determined by SDS-PAGE

Endotoxin
< 0.1 EU per μg of the protein as determined by the LAL method

Activity
Measured in a cell proliferation/survival assay using 3T3‑L1 mouse embryonic fibroblast adipose-like cells. The ED50 for this effect is 0.4‑1.6 μg/mL.

Storage
-80 °C, avoid repeated freeze-thaw cycles.

Storage Buffer
PBS