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Description
The 100 bp DNA Ladder is suitable for sizing double-stranded DNA fragments from 100-2000 bp. The ladder is prepared from a plasmid containing repeats of a 100 bp DNA fragment. Agarose gel analysis must show that bands between 100-1500 bp are distinguishable after ethidium bromide staining. The 1000 bp band must be more intense than any other ladder band except the 2000 bp band. This ladder is premixed with Gel Loading Dye, Blue (6X) for direct gel electrophoresis.
Application
Determination of the molecular weight of nucleic acids for analyzing restriction digests and PCR products. The DNA Ladder, 100-2000 bp was not designed for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
Effective Size Range: 100 bp to 2000 bp
Concentration and Usage: 0.1 μg/μl, recommended loading amount is 5 μl per lane.
Stability: This product is stable at room temperature for at least 1 month and at 4°C for at least 3 months. Recommended Storage Condition: -20°C
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