MagnifiQ™ 16 Plasmid Mini instant kit

Description:
MagnifiQ 16 Plasmid Mini instant kit is designed for automated magnetic isolation of high-copy plasmid DNA from bacterial culture in a 16-samples-per-plate format. It contains ready-to-use, reagent-filled plates and all necessary consumables. Sample preparation is based on alkaline lysis with the LySee color system for easy visual control of lysis progress. According to the manufacturer, this system prevents common handling errors such as incomplete cell resuspension, inefficient cell lysis or incomplete precipitation of unwanted cell components. The MagnifiQ product series is based on automated nucleic acid isolation using magnetic beads. According to the manufacturer, this approach significantly shortens working time and reduces the risk of error compared to manual methods. The isolated material is, according to the manufacturer, suitable for downstream analysis by PCR and real-time PCR as well as for sequencing. The manufacturer also states that only a few minutes of manual work are needed for sample preparation, with the rest of the procedure carried out by the automatic extraction device, and that no initial preparation of buffers is required: the extracted material is available after about 30 minutes.

Features:

• Automated magnetic isolation of high-copy plasmid DNA using magnetic beads
• 16 samples per plate format
• XP-P plates pre-filled with reagents
• LySee color system for visual control of alkaline lysis
• No buffer preparation required for the isolation itself
• Protocol time approx. 30 min
• Sample size up to 3 ml of bacterial culture
• Elution volume 60 µl
• Elution solution: Tris buffer
• Binding capacity 30 µg DNA
• Tested with Allsheng Auto-Pure Mini, Auto-Pure S32 and Auto-Pure 32A devices. According to the manufacturer, this does not preclude use with other devices
• For research use only

Applications:

• High-copy plasmid DNA isolation from bacterial culture: 1.5–3 ml of overnight culture is used for processing
• DNA preparation for PCR, real-time PCR and sequencing

Kit contents:

• XP-P extraction plates
• L1 cell suspension solution (storage at 2–8 °C)
• L2 lysis solution
• L3 neutralizing solution
• Tip comb 8
• Protective film

Specification:

Protocol files: