PfAGO (Pfu Argonaute)

Description:
Prokaryotic Argonaute (pAGO) is the key protein in the host defense system by mediating nucleic acid molecules. As the DNA-guided endonuclease from hyperthermophilic archaeon Pyrococcus furiosus, pfAGO protein targets cognate DNA without the requirements of a PAM (protospacer adjacent motif) or PFS (protospacer flanking site) on the target sequence, which largely extends the application in selection of available target DNA sequence. Besides, compared to CRISPR/Cas systems, PfAGO endonuclease exploits guide DNA rather than guide RNA in stimulating target DNA cleavage, which is more convenient for in vitro use as guide DNA is more stable and easier, cost-saver to synthesis than guide RNA. These properties enable pfAGO as a programmable DNA endonuclease guided by short guide DNAs in efficient, rapid and cost-effective Nucleic Acid Detection systems as well as in versatile Synthetic Biology platforms.

Purity is greater than 95% as determined by SDS-PAGE and FPLC.

The protein is supplied at a concentration of 250 pmol, 50 µl (5 µM).

Figure 1 shows a schematic representation of DNA cleavage directed by ssDNA using PfAGO. The results of DNA cleavage using PfAGO are shown in Figure 2.

Application:

• Nucleic Acid Detection
• Cancer Diagnostics
• Synthetic Biology
• Genetic Screening
• Source:
  PfAgo gene from Pyrococcus furiosus (NCBI-Protein ID: AAL80661) expressed in E. coli

Storage:
-20 °C