TtAGO (Tth Argonaute)

Description:
Prokaryotic Argonaute (pAGO) is the pivotal enzyme in the host defense system by mediating nucleic acid molecules. As the DNA-guided endonuclease from eubacterium Thermus thermophilus, TtAGO protein targets cognate DNA without the restriction of a PAM (protospacer adjacent motif) or PFS (protospacer flanking site) on the target sequence, expanding the selection range of available target DNA sequence. Besides, compared with CRISPR/Cas systems, TtAGO have remarkable advantages as TtAGO endonuclease utilizes guide DNA rather than guide RNA in directing target DNA cleavage, which is more convenient for in vitro applications as guide DNA is more stable in ambient temperature, easier and cost-saving to synthesize than guide RNA. These capacities enable TtAGO as a powerful programmable DNA endonuclease directed by short guide DNAs in efficient Nucleic Acid Detection systems as well as in versatile Synthetic Biology platforms.

The enzyme's purity is greater than 95%, as determined by SDS-PAGE and FPLC.

Activity: Purified 370 bp PCR product of dsDNA cleaved after 1 hour of reaction at 83°C. Figure 1 shows a schematic representation of DNA cleavage directed by ssDNA using TtAGO. The results of DNA cleavage using TtAGO are shown in Figure 2.

Application:

• Nucleic Acid Detection
• Cancer Diagnostics
• Genetic Screening
• Synthetic Biology

Source:
TtAgo gene from Thermus thermophilus (NCBI Reference Sequence: WP_011174533.1) expressed in E. coli.

Storage:

-20°C 

Size: 

200 pmol, 200 ul (1 uM) (cat.n. TtAGO-10)

1 nmol, 200 ul (5 uM) (cat.n.. TtAGO-50)