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- Nucleic Acid Analysis
- Protein Analysis
- Biochemical Reagents
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Enzymes
- Thermophilic DNA Polymerases
- Mesophilic DNA Polymerases
- Restriction Endonucleases
- Reverse Transcriptase and RNA Polymerases
- DNA/RNA Ligases
- RNases
- Proteases
- Nucleases
- Kinases
- Phosphatases and Sulfurylases
- DNA Repair Proteins
- Single-Stranded DNA Binding Proteins
- Chaperon Proteins and Disulfide Bond Isomerase
- Others
- Gene editing
- Molecular cloning
- Clinical diagnostics
- Human Identification STR kits
- Laboratory instruments
- Software
- A&A Biotechnology
News
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Kapras Day 2025
We would like to invite you to the 23rd Kapras Day on the topic of "Clinical Genetics," which will take place on Wednesday, February 26, 2025, in the Congress Hall of Hotel Olšanka in Prague. We loo...
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Description:
BioDynami DNA/RNA quantification kits are designed for accurate quantification of DNA and RNA samples across a wide concentration range using the Qubit™ fluorometer.
The ssDNA Quantification Kit is developed for quantification of single-stranded DNA and includes dye, dilution buffer, and two ssDNA standards. The assay is accurate for DNA concentrations ranging from 50 pg/µL to 200 ng/µL, based on comparison of the data with standards, as shown in the graph below.
The kit detects ssDNA using a fluorescent dye, which enables sensitive quantification of single-stranded DNA, including ssDNA viruses, synthetic ssDNA, the first stage of cDNA synthesis, denatured DNA, and bisulfite-converted DNA, etc.
Protocol:
The test procedure is simple – just dilute the ssDNA dye with the ssDNA dilution buffer, add the DNA sample (volume 1-20 μL), and then measure the concentration using the Qubit™ fluorometer.
Features:
- Optimized for use with the Qubit™ fluorometer
- Uses the Qubit™ ssDNA assay setting
- Linear range: 1–200 ng dsDNA
- Cost-effective – reduces costs by 50%
The kit reduces analysis costs by nearly 50% (compared to the catalog price of the Qubit ssDNA product from Thermo Fisher Scientific).
DNA Selectivity and Sensitivity:
For testing, samples with and without RNA contamination were used in a series of input DNA. The comparison results with the kit from Thermo Fisher Scientific confirm the high accuracy and reliability of the BioDynami kit.
Effect of Contaminants:
The tests are well-tolerated by common contaminants such as salt, solvents and detergents as shown in the table below.
Qubit™ is a registered trademark of Thermo Fisher Scientific.
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