Library/DNA Size Selection Kit (250-350 bp, Magnetic Beads)

Description:

The Library Size Selection Kit series from the U.S.-based company BioDynami is designed for NGS library preparation for both short- and long-read sequencing. This kit is ideal for size selection of NGS libraries intended for Illumina PE100 sequencing with insert sizes of 100–200 bp.

The workflow supports both double-sided size selection for short reads and single-sided selection for long reads. Thorough and high-quality library preparation is crucial for achieving accurate and reliable sequencing results. Precise size selection enhances sequencing efficiency, improves data quality, and reduces costs.

The magnetic bead technology utilizes paramagnetic particles, also known as SPRI beads (Solid Phase Reversible Immobilization), which enable reversible and selective DNA binding. DNA fragments can be selectively captured and purified by modifying the properties of these beads. The magnetic beads efficiently separate DNA from contaminants and unwanted components such as buffer components, enzymes, proteins, salts, dNTPs, primers, and adapters. The kit's patented reagents enhance yield, selectivity, and reproducibility, ensuring optimal performance.

Procedure:
The double-clean-up DNA selection procedure is illustrated in the image below. The first bead-binding step, referred to as right-side clean-up, removes large DNA fragments. These large fragments bind to the beads and are then discarded. The desired DNA fragments in the supernatant are transferred to a new well, and new beads are added for the second bead-binding, called left-side clean-up. The double-size-selected DNA fragments are eluted after ethanol washing.

Benefits:

• Completion within 20 minutes
• No column required
• No gel purification required
• No centrifugation required

Package Size:

24 runs (Cat. No. 20104S)

96 runs (Cat. No. 20104L)