Proteinase K

Description:

Proteinase K is a serine protease with broad proteolytic activity, ideal for the efficient degradation of proteins during nucleic acid isolation and PCR product purification. The solution is ready to use immediately and, thanks to recombinant production, provides high activity even under variable conditions. Proteinase K exhibits enhanced proteolytic activity in the presence of SDS and urea. The recombinant form offers high performance across a wide pH and temperature range. The recommended working concentration is 50–100 μg/ml.

Specifications:

• Ready-to-use solution: 20 mg/ml
• High activity: Performs well between 20–60 °C (optimal efficiency at 50–55 °C)
• Broad pH range: Optimal activity from pH 4.5–12.0, with a peak between pH 7.5–11.5
• Purity: Free from detectable DNase, RNase, endonuclease, and exonuclease activity

Applications:

• Inactivation of DNases, RNases, and other enzymes in reactions
• Protein removal during isolation of genomic DNA and RNA from cells or tissues
• Plasmid DNA extraction
• Improving cloning efficiency of PCR fragments
• PCR product purification

Product sizes:

1 ml (Cat. No. 40109S)
5 ml (Cat. No. 40109L)