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XXXV. Izakovičov memoriál 2025
We are pleased to announce our participation in the prestigious XXXV. Izakovič Memorial 2025, which will take place on October 8–10, 2025 at the Grandhotel Praha, Tatranská Lomnica. The Izakovič Memo...
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1st Czechoslovak Congress of Medical Genetics 2025
In the spring, we will participate in the 1st Czechoslovak Congress of Medical Genetics, which will take place from April 2–4, 2025, at the Cultural and Congress Center Elektra in the spa town of Luha...
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RANK 2025
Visit us at the 19th edition of the RANK 2025 conference, which will take place on March 19th and 20th at the Zlatá Štika Hotel in Pardubice. The conference is organized by the Czech Society of Clinic...
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Description:
Originally developed for the micropropagation of walnut (Juglans spp.) from nodal explants, DKW Medium is now widely used for the in vitro cultivation of woody species, herbs, and recalcitrant plants. Its distinct formulation features lower nitrogen content and higher sulfate levels, supporting plant vitality and development under tissue culture conditions.
Features:
- Complete mineral and vitamin composition for robust explant growth
- Easy to prepare and versatile for multiple plant types
- Supports shoot proliferation and regeneration from callus
- Optimized for woody plants and challenging species (e.g., turmeric, walnut)
Recommended use:
- Micropropagation of woody and herbaceous plants
- Shoot induction and regeneration from callus
- Suitable for recalcitrant species requiring tailored media composition
Preparation Instructions:
- Dissolve 5.36 g of DKW powder in 800 ml of distilled water
- Add 30 g sucrose and PPM™ (1–2 ml/l)
- Adjust volume to 1 liter with distilled water
- Adjust pH to 5.4–5.8
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Add a gelling agent:
Agar (6–8 g/l) or - Gellan gum (3–4 g/l)
- Sterilize in an autoclave for 20 minutes at 121 °C, 15 psi
- Medium is ready for use
Applications:
- In vitro propagation of woody plants, herbs, and difficult-to-root species
- Shoot multiplication and callus regeneration
- Enhancing success rates for specialized plant tissue culture protocols
Video tutorial:
Available sizes:
1 L (Cat. No. DKW1L)
10 L (Cat. No. DKW10L)
50 L (Cat. No. DKW50L)
100 L (Cat. No. DKW100L)
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